4E-BP1 rabbit pAb Antibody

SKU: orb767955

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Description

Rabbit polyclonal antibody to 4E-BP1

Images & Validation

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Item 1 of 2

Tested Applications	ELISA, IF, IHC, WB
Dilution range	Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.
Reactivity	Human, Mouse, Rat

Key Properties

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Clonality	Polyclonal
Isotype	IgG
Immunogen	The antiserum was produced against synthesized peptide derived from human 4E-BP1. AA range:30-79
Molecular Weight	15
Purification	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
Concentration	1 mg/ml
Disclaimer	For research use only

Alternative Names

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Anti-EIF4EBP1 antibody, anti-Eukaryotic translation initiation factor 4E-binding protein 1 antibody, anti-4E-BP1 antibody, anti-eIF4E-binding protein 1 antibody, anti-Phosphorylated heat- and acid-stable protein regulated by insulin 1 antibody, anti-PHAS-I antibody

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Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using 4E-BP1 Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from Jurkat cells, treated with Insulin 0.01U/ml 15', using 4E-BP1 Antibody. The lane on the right is blocked with the synthesized peptide.

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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Immunofluorescence

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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