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AMPKβ1 (phospho Ser182) rabbit pAb Antibody
SKU: orb769610
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Description
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| Tested Applications | ELISA, IF, IHC, WB |
|---|---|
| Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications. |
| Reactivity | Human, Monkey, Mouse, Rat |
Key Properties
−| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human AMPK beta1 around the phosphorylation site of Ser181. AA range:147-196 |
| Molecular Weight | 33 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
| Concentration | 1 mg/ml |
| Disclaimer | For research use only |
Alternative Names
−Anti-PRKAB1 antibody, anti-AMPK antibody, anti-5'-AMP-activated protein kinase subunit beta-1 antibody, anti-AMPK subunit beta-1 antibody, anti-AMPKb antibody
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AMPKβ1 (phospho Ser182) rabbit pAb Antibody [orb764134]
ELISA, IF, IHC, WB
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100 μl, 50 μl
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Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using AMPK beta1 (Phospho-Ser181) Antibody.
Western blot analysis of lysates from SH-SY5Y cells, primary antibody was diluted at 1:1000, 4°C over night.
Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Tris-EDTA, pH9.0 was used for antigen retrieval. 2 Antibody was diluted at 1:200 (4°C overnight.3, Secondary antibody was diluted at 1:200 (room temperature, 45min).
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
AMPKβ1 (phospho Ser182) rabbit pAb Antibody (orb769610)
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