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Angiopoietin 4 Polyclonal Antibody
SKU: orb1421318
Description
Research Area
Cancer, Epigenetics
Images & Validation
−Item 1 of 2
| Tested Applications | ELISA, IF, IHC-Fr, IHC-P, WB |
|---|---|
| Reactivity | Porcine, Rat |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Disclaimer | For research use only |
Alternative Names
−Angiopoietin-4, ANG-4, Angiopoietin-3, ANG-3, ANGPT4, ANG3, ANG4
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Sample: Lung (Mouse) Lysate at 30 ug. Lung (Rat) Lysate at 30 ug. Primary: Anti- Anti-ANGPT3/ANG3/ANGPT4/ANG4 at 1/400 dilution. Secondary: HRP conjugated Goat-Anti-rabbit IgG at 1/3000 dilution. Predicted band size: 57kD. Observed band size: 57kD.
Tissue/cell: mouse tumor tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-ANGPT3/ANG3/ANGPT4/ANG4 Polyclonal Antibody, Unconjugated 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
Quick Database Links
UniProt
UniProt Details
− No UniProt data available
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IHC-Fr
Immunohistochemistry Frozen
IF
Immunofluorescence
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
Angiopoietin 4 Polyclonal Antibody (orb1421318)
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