CD16 Antibody-Biotin labled

SKU: orb1294191

Description

The CD16 Antibody-Biotin labled is suitable for FC, IF. It is a Monoclonal, Biotin conjugated antibody which raised against Native purified human CD16.. Purification: The antibody was purified by affinity chromatography.

Images & Validation

−

Tested Applications	FC, IF
Dilution range	10 μl / assay
Reactivity	Human
Application Notes	1. Take 100 μl peripheral blood anticoagulated by EDTA and add to the bottom of 5 ml tube. 2. Add 10 μl labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 ml RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 ml PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add appropriate amount of fluorescent-labeled Streptavidin and incubate for 20 minutes away from light at room temperature. 7. Repeat step 5. 8. Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured). Read more...

Key Properties

−

Antibody Type	Primary Antibody
Host	Mouse
Clonality	Monoclonal
Clone No.	CB16
Immunogen	Native purified human CD16.
Purification	The antibody was purified by affinity chromatography.
Conjugation	Biotin

Storage & Handling

−

Storage	Shipped and store at 4°C for one year. Do not freeze.
Form/Appearance	Mouse IgG1. Liquid in PBS, pH 7.3, 0.2% rAlbumin, and 0.02% sodium azide.
Disclaimer	For research use only

Alternative Names

−

CD16B; FCG3; FCGR3; IGFR3; Low affinity immunoglobulin gamma Fc region receptor III-B; Fc-gamma RIII-beta; Fc-gamma RIII; Fc-gamma RIIIb; FcRIII; FcRIIIb; FcR-10; IgG Fc receptor III-1; CD antigen CD16b

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

Quick Database Links

UniProt

O75015

NCBI Gene ID

2215

UniProt Details

−

No UniProt data available

NCBI Gene Details

−

No NCBI Gene data available

Documents Download

Datasheet

Product Information

Download

Request a Document

Protocol Information

Flow Cytometry

View Protocol

Immunofluorescence

View Protocol

Find More Protocols