Anti-CD45RA [OTH-74D4]

SKU: orb758904

Description

Rabbit monoclonal antibody to CD45RA

Images & Validation

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Tested Applications	FC
Reactivity	Human

Key Properties

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Host	Mouse
Clonality	Monoclonal
Isotype	Mouse IgG
Clone No.	OTH-74D4
Immunogen	This antibody was raised by immunising mice with an EBV-transformed B cell line from a patient with MHC class II deficiency.
Target	CD45RA
Purity	Purified
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	PBS with 0.02% Proclin 300.
Concentration	1 mg/ml
Disclaimer	For research use only

Alternative Names

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PTPRC; Receptor-type tyrosine-protein phosphatase C; Leukocyte common antigen; L-CA; T200; CD45; LY5; T200 glycoprotein; T200 leukocyte common antigen; CD45RA isoform; OTH 74D4

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Flow cytometry using the Anti-CD45RA antibody OTH-74D4. Paraformaldehyde fixed Jurkat cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (orb256458; isotype control, black line) or the rabbit IgG version of OTH-74D4 (orb758904, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of Jukat cells with anti-CD45RA OTH-74D4. Immunofluorescence analysis of paraformaldehyde fixed Jukat cells on Shi-fix™ cover-slips stained with the chimeric rabbit IgG version of OTH-74D4 (orb758904) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb758904, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (orb256458) followed by staining with Alexa Fluor® 488 secondary antibody.

Quick Database Links

Gene Symbol

CD45RA

UniProt

P08575

UniProt Details

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No UniProt data available

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Protocol Information

Flow Cytometry

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