F4/80 Antibody

Western blot analysis of MTMR9 expression in Hela (A), A549 (B), mouse brain (C), rat brain (D) whole cell lysates. (Predicted band size: 53; 63 kD; Observed band size: 63 kD)

Immunofluorescent analysis of F4/80 staining in Raw264.7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).