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F4/80 Antibody
SKU: orb665909
Description
Research Area
Epigenetics, GPCR, Immunology, Neuroscience, Signaling Pathways
Images & Validation
−Item 1 of 2
| Tested Applications | IF, WB |
|---|---|
| Dilution Range | WB: 1-500-2000, IHC-P: 1-50-200 |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Immunogen | Recombinant protein of human F4/80. The exact sequence is proprietary. |
| Purification | The antibody was purified by immunogen affinity chromatography. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−EMR1; TM7LN3; EGF-like module-containing mucin-like hormone receptor-like 1; EGF-like module receptor 1; EMR1 hormone receptor; Adhesion G protein-coupled receptor E1; F4/80
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![Anti-F4/80 [Cl:A3-1 (recombinant version)]](/images/pub/media/catalog/product/NewWebsite/35/orb411543_1.png)
![Anti-F4/80 [Cl:A3-1 (recombinant version)]](/images/pub/media/catalog/product/NewWebsite/35/orb411543_2.png)
![Anti-F4/80 [Cl:A3-1 (recombinant version)]](/images/pub/media/catalog/product/NewWebsite/35/orb411543_3.png)
![Anti-F4/80 [Cl:A3-1 (recombinant version)]](/images/pub/media/catalog/product/NewWebsite/35/orb411544_1.png)
![Anti-F4/80 [Cl:A3-1 (recombinant version)]](/images/pub/media/catalog/product/NewWebsite/35/orb411544_2.png)
![Anti-F4/80 [Cl:A3-1 (recombinant version)]](/images/pub/media/catalog/product/NewWebsite/35/orb411544_3.png)
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Western blot analysis of MTMR9 expression in Hela (A), A549 (B), mouse brain (C), rat brain (D) whole cell lysates. (Predicted band size: 53; 63 kD; Observed band size: 63 kD)
Immunofluorescent analysis of F4/80 staining in Raw264.7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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Protocol Information
WB
Western Blot (IB, immunoblot)
IF
Immunofluorescence
F4/80 Antibody (orb665909)
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