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CD148 Antibody

SKU: orb154402

Description

Mouse monoclonal antibody to CD148 which is also known as DEP-1, HPTP-eta, and PTP receptor type J (PTPRJ), is an enzyme that removes phosphate groups covalently attached to tyrosine residues in proteins. It is widely expressed in tissues, particularly those forming epithelioid monolayers. CD148 can be also used as marker of the most mature human thymocytes, and leukemic cells corresponding to this stadium of thymocyte differentiation.

Research Area

Epigenetics

Images & Validation

Tested ApplicationsFC
ReactivityHuman
Application Notes
Flow cytometry: Recommended dilution: 1-5 μg/ml.

Key Properties

Antibody TypePrimary Antibody
ClonalityMonoclonal
IsotypeMouse IgG2b
Clone No.MEM-CD148/05
ImmunogenHuman recombinant CD148 (amino acids 1-444)
TargetCD148
PurificationPurified by protein-A affinity chromatography.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesPhosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Concentration1 mg/ml
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

HPTP-eta, DEP-1, PTPRJ, DEP1, SCC1, HPTPeta, R-PTP-ETA

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CD148 Antibody

Separation of human neutrophil granulocytes (red-filled) from lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD148 (MEM-CD148/05) purified antibody (concentration in sample 2 μg/ml, GAM APC).

CD148 Antibody

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD148 (MEM-CD148/05) purified antibody (concentration in sample 2 μg/ml, GAM APC).

UniProt Details

No UniProt data available

NCBI Gene Details

No NCBI Gene data available

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Protocol Information

FC
Flow Cytometry
View Protocol

CD148 Antibody (orb154402)

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0.1 mg
$ 280.00
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