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MKK4 (pT261) Antibody

SKU: orb2651298

Description

Rabbit polyclonal antibody to MKK4 (pT261)

Images & Validation

Tested ApplicationsIF, IHC, WB
Dilution RangeWB: WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500), IF: WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)
ReactivityHuman, Mouse, Rat, Zebrafish

Key Properties

HostRabbit
ClonalityPolyclonal
Clone No.MAP2K4
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

JNKK1; MEK4; MKK4; PRKMK4; SEK1; SERK1; SKK1; Dual specificity mitogen-activated protein kinase kinase 4; MAP kinase kinase 4; MAPKK 4; JNK-activating kinase 1; MAPK/ERK kinase 4; MEK 4; SAPK/ERK kinase 1; SEK1; Stress-activated protein kinase kinase 1; SAPK kinase 1; SAPKK-1; SAPKK1; c-Jun N-terminal kinase kinase 1; JNKK

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MKK4 (pT261) Antibody

Western blot analysis of MKK4 (pT261) expression in zebrafish (A) whole cell lysates.

MKK4 (pT261) Antibody

Immunohistochemical analysis of MKK4 (pT261) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

MKK4 (pT261) Antibody

Immunofluorescent analysis of MKK4 (pT261) staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

UniProt Details

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
IF
Immunofluorescence
View Protocol

MKK4 (pT261) Antibody (orb2651298)

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Available Sizes

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50 μl
$ 360.00
DispatchUsually dispatched within 5-10 working days
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