APOD Antibody / Apolipoprotein D

SKU: orb699652

Description

Lipids, such as phospholipids, triacylglycerols and cholesterol, are weakly soluble in aqueous solution and therefore are transported by circulation as components of lipoproteins. Lipoproteins are globular particles that consist of a non-polar core of triacylglycerols and cholesteryl esters surrounded by phospholipid, cholesterol and an amphiphilic coating of protein, known as apolipoproteins (apo). These complexes allow the dissolution and shuttling of their non-polar lipid components. At least nine different apolipoproteins are distributed in significant amounts in different human lipoproteins. Apolipoprotein D (apoD) is a member of the lipocalin superfamily of transporter proteins that bind small hydrophobic molecules, including arachidonic acid (AA). The ability of apoD to bind AA implicates it in pathways associated with membrane phospholipid signal transduction and metabolism. apoD expression has been shown to correlate both with cell cycle arrest and with prognosis in several types of malignancy, including central nervous system astrocytomas and medulloblastomas.

Images & Validation

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Tested Applications	IHC-P
Dilution range	Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT
Reactivity	Human
Application Notes	Optimal dilution of the APOD antibody should be determined by the researcher.

Key Properties

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Antibody Type	Primary Antibody
Host	Mouse
Clonality	Monoclonal
Isotype	Mouse IgG2c, kappa
Clone No.	APOD/3414
Immunogen	A portion of amino acids 21-119 from the human protein was used as the immunogen for the APOD antibody.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Disclaimer	For research use only

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IHC staining of FFPE human liver with APOD antibody. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using APOD antibody. These results demonstrate the foremost specificity of the APOD/3414 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE analysis of purified, BSA-free APOD antibody as confirmation of integrity and purity.

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UniProt

P05090

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No UniProt data available

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Protocol Information

IHC-P

Immunohistochemistry Paraffin

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