Aquaporin 4 Antibody

SKU: orb67574

Description

Rabbit Anti-Rat Aquaporin 4 Polyclonal

Research Area

Cell Biology, Neuroscience, Pharmacology & Drug Discovery

Images & Validation

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Item 1 of 2

Tested Applications	ICC, IF, IHC, WB
Dilution range	WB (1:2000), IHC-P (1:200), ICC/IF (1:400)
Reactivity	Human, Mouse, Rat
Application Notes	0.5 µg/ml of SPC-505 was sufficient for detection of aquaporin 4 in 10 µg of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

Key Properties

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Host	Rabbit
Clonality	Polyclonal
Immunogen	Produced against the C-terminal peptide (Sequence N-CTKGSYMEVEDNRSQVETED) of rat aquaporin 4
Target	Aquaporin 4
Molecular Weight	50kDa
Purification	Protein A Purified
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	PBS, 50% glycerol, 0.09% sodium azide *Storage buffer changes when conjugated
Concentration	1 mg/ml
Disclaimer	For research use only

Alternative Names

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Aquaporin 4, Aquaporin-4, AQP4, AQP-4, AQP 4, AQP4_HUMAN, Aquaporin type 4, Aquaporin4, HMIWC 2, HMIWC2, Mercurial insensitive water channel, Mercurial-insensitive water channel, MGC22454, MIWC, WCH 4, WCH4

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Immunohistochemistry analysis using Rabbit Anti-Aquaporin 4 Polyclonal Antibody. Tissue: kidney tissue. Species: Rat. Fixation: 4% paraformaldehyde-lysine-periodate. Primary Antibody: Rabbit Anti-Aquaporin 4 Polyclonal Antibody at 1:200 for 75 min at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) for 1 hour at RT. 4 μm cryostat sections.

Western blot analysis of Rat kidney inner medullary homogenates showing detection of Aquaporin 4 protein using Rabbit Anti-Aquaporin 4 Polyclonal Antibody. Primary Antibody: Rabbit Anti-Aquaporin 4 Polyclonal Antibody at 1:2000.

Quick Database Links

Gene Symbol

Aquaporin 4

UniProt

P47863

NCBI Gene ID

25293

RefSeq (Protein):NP_036957.1

UniProt Details

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No UniProt data available

NCBI Gene Details

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No NCBI Gene data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_036957.1

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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Immunofluorescence

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ICC

Immunocytochemistry

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