Arginase 1 Antibody

SKU: orb749618

Description

Recognizes a protein of 35-38kDa, which is identified as Arginase 1 (ARG1). Arginase is a manganese metallo-enzyme that catalyzes the hydrolysis of arginine to generate ornithine and urea. Arginase I and II are isoenzymes which differ in subcellular localization, regulation, and possibly function. Arginase I is a cytosolic enzyme, which is expressed mainly in the liver as part of the urea cycle, whereas arginase II is a mitochondrial protein found in a variety of tissues. Antibody to ARG-1 labels hepatocytes in normal tissues and granulocytes in peripheral blood. ARG-1 is a sensitive and specific marker for identification of hepatocellular carcinoma.

Research Area

Epigenetics, Immunology

Images & Validation

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Tested Applications	IHC-P, WB
Dilution range	Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 2-4ug/ml for 30 min at RT
Reactivity	Human
Application Notes	Optimal dilution of the Arginase 1 antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

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Antibody Type	Primary Antibody
Host	Mouse
Clonality	Monoclonal
Isotype	Mouse IgG3, kappa
Clone No.	ARG1/1125
Immunogen	A recombinant fragment from amino acids 1-150 of human ARG1 was used as the immunogen for the Arginase 1 antibody.
Purification	Protein G affinity chromatography
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Disclaimer	For research use only

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Western blot analysis of A) partial recombinant ARG1 protein and B) human liver lysate using Arginase 1 antibody (ARG1/1125). Predicted molecular weight ~35 kDa.

IHC: Formalin-fixed, paraffin-embedded human hepatocellular carcinoma stained with Arginase 1 antibody (ARG1/1125). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT prior to testing.

Analysis of HuProt (TM) microarray containing more than 19, 000 full-length human proteins using Arginase 1 antibody (clone ARG1/1125). These results demonstrate the foremost specificity of the ARG1/1125 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE analysis of purified, BSA-free Arginase 1 antibody (clone ARG1/1125) as confirmation of integrity and purity.

Western blot analysis of human liver lysate using Arginase 1 antibody (ARG1/1125). Predicted molecular weight ~35 kDa.

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UniProt

P05089

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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