C-jun Rabbit Polyclonal Antibody

Blank control (black line): Hela. Primary Antibody (green line): Rabbit Anti-C-jun antibody (orb1816949), dilution: 1 ug/Test, Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF488, dilution: 0.5 ug/Test. Isotype control (orange line): Normal Rabbit IgG, Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C, The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Measured by its binding ability in a indirect ELISA. Immobilized Human C-jun protein, His & MBP Tag at 2 µg/mL (100 µl/well) can bind Rabbit Anti-Human C-jun Antibody, the EC50 is 180.9 ng/ml.

Measured by its binding ability in a indirect ELISA. Immobilized Human C-jun protein, His & MBP Tag at 2 µg/mL (100 µl/well) can bind Rabbit Anti-Human C-jun Antibody, the EC50 is 27.15 ng/ml.