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Casp12 Antibody
SKU: orb1239263
Featured
Description
Research Area
Cancer Biology, Cell Biology
Images & Validation
−Item 1 of 7
| Tested Applications | ELISA, IF, IHC-P, WB |
|---|---|
| Reactivity | Human, Mouse, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Caspase-12 antibody was raised against a synthetic peptide corresponding to 16 amino acids within the large cleavage product of murine caspase-12.The immunogen is located within amino acids 170 - 220 of Caspase-12. |
| Target | Casp12 |
| Molecular Weight | Predicted: 46 kDa Observed: 46 kDa |
| Purification | Caspase-12 (Large) Antibody is affinity chromatography purified via peptide column. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Caspase-12 Antibody is supplied in PBS containing 0.02% sodium azide. (Large) |
| Concentration | 1 mg/mL |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Caspase-12 (Large) Antibody: Caspase-12, CASP-12
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Immunofluorescence Validation of Caspase-12 in Mouse Heart. Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse heart labeling caspase-12 with orb1239263 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI antibody (blue).
Immunohistochemistry Validation of Caspase-12 in Human Colon. Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-Caspase-12 antibody (orb1239263) at 1 µg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Immunohistochemistry Validation of Caspase-12 in Mouse Brain. Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Caspase-12 antibody (orb1239263) at 1 µg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Immunohistochemistry Validation of Caspase-12 in Rat Brain. Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Caspase-12 antibody (orb1239263) at 1 µg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
WB Validation in Human and Mouse Heart. Loading: 15 µg of lysate Antibodies: Caspase-12, orb1239263, 0.5 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution. Lane A: Human heart Lane B: Mouse heart.
WB Validation in Human Tissues. Loading: 15 µg of lysate Antibodies: Caspase-12, orb1239263, 1 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
WB Validation in Rat Tissues. Loading: 15 µg of lysate Antibodies: Caspase-12, orb1239263, 1 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IF
Immunofluorescence
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
Casp12 Antibody (orb1239263)
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