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Caspase-9 (phospho Ser196) rabbit pAb Antibody

SKU: orb770615
FeaturedFeatured Product

Description

Rabbit polyclonal antibody to Caspase-9 (phospho-Ser196)

Images & Validation

Tested ApplicationsELISA, IF, IHC, WB
Dilution RangeWestern Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.
ReactivityHuman, Mouse, Rat

Key Properties

ClonalityPolyclonal
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from human Caspase 9 around the phosphorylation site of Ser196. AA range:162-211
Molecular Weight50
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesPBS with 0.02% sodium azide and 50% glycerol pH 7.4.
Concentration1 mg/ml
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

Anti-CASP9 antibody, anti-MCH6 antibody, anti-Caspase-9 antibody, anti-CASP-9 antibody, anti-Apoptotic protease Mch-6 antibody, anti-Apoptotic protease-activating factor 3 antibody, anti-APAF-3 antibody, anti-ICE-like apoptotic protease 6 antibody, anti-ICE-LAP6 antibody

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Caspase-9 (phospho Ser196) rabbit pAb Antibody

Western Blot analysis of 293 cells using Phospho-Caspase-9 (S196) Polyclonal Antibody diluted at 1:1000.

Caspase-9 (phospho Ser196) rabbit pAb Antibody

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Caspase 9 (Phospho-Ser196) Antibody.

Caspase-9 (phospho Ser196) rabbit pAb Antibody

Western blot analysis of lysates from 293 cells treated with Calyculin 50nM 30', using Caspase 9 (Phospho-Ser196) Antibody. The lane on the right is blocked with the phospho peptide.

Caspase-9 (phospho Ser196) rabbit pAb Antibody

Immunohistochemical analysis of paraffin-embedded human Colon cancer. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

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Protocol Information

WB
Western Blot (IB, immunoblot)
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IHC
Immunohistochemistry
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IF
Immunofluorescence
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ELISA
Enzyme-linked Immunosorbent Assay (EIA)
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Caspase-9 (phospho Ser196) rabbit pAb Antibody (orb770615)

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50 μl
$ 250.00
100 μl
$ 390.00
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