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CAT Antibody

SKU: orb1248005

Description

CAT Antibody

Research Area

Cell Biology

Images & Validation

Tested ApplicationsELISA, WB
ReactivityHuman, Mouse
Predicted ReactivityCanine
Application Notes
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 60kDa band observed in Human and Mouse Kidney and Liver lysates (calculated MW of 59.8kDa according to Human NP_001743.1 and Mouse NP_033934.2 ). Recommended concentration: 0.1-0.3µg/mL. Primary incubation 1 hour at room temperature.

Key Properties

Antibody TypePrimary Antibody
HostGoat
ClonalityPolyclonal
ImmunogenThe immunogen for this antibody is: C-DKYNAEKPKNAIHT
TargetCAT
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration500 ug/mL
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

CAT, catalase , HGNC:1516, MGC138422, MGC138424

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CAT Antibody

orb1248005 (0.1 ug/ml) staining of Human Kidney (A) and Liver (B) and Mouse Kidney (C) and Liver (D) lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

CAT Antibody

orb1248005 (2 ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. This data is from a previous batch, not on sale.

CAT Antibody

orb1248005 (2 ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. This data is from a previous batch, not on sale.

UniProt Details

No UniProt data available

NCBI Reference Sequences

Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product

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Product Information
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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

CAT Antibody (orb1248005)

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Available Sizes

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0.1 mg
$ 920.00
DispatchUsually dispatched within 5-10 working days
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