CD47 Antibody / IAP

SKU: orb607105

Description

This antibody reacts with Ig domain of CD47 protein. CD47, originally named integrin-associated protein (IAP), is a 50kDa protein containing five membrane-spanning sequences and a short cytoplasmic tail. CD47 plays a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. It is important in memory formation and synaptic plasticity in the hippocampus. CD47 may play a role in membrane transport and/or integrin dependent signal transduction.

Images & Validation

−

Item 1 of 3

Tested Applications	FACS, IF, IHC-P
Dilution range	Immunofluorescence/Immunocytochemistry: 1-2ug/ml,Flow cytometry: 1-2ug/10^6 cells,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)
Reactivity	Human
Application Notes	Optimal dilution of the CD47 antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

−

Antibody Type	Primary Antibody
Host	Mouse
Clonality	Monoclonal
Isotype	Mouse IgG1, kappa
Clone No.	CDLA47-1
Immunogen	Amino acids 18-135 from the human protein were used as the immunogen for the CD47 antibody.
Purification	Protein G affinity chromatography
Conjugation	Unconjugated

Storage & Handling

−

Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Disclaimer	For research use only

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

IHC staining of FFPE human prostate carcinoma with CD47 antibody (clone CDLA47-1). HIER: boil tissue sections in pH6, 10 mM citrate buffer, for 10-20 min and allow to cool before testing.

Flow cytometry testing of human MCF7 cells with CD47 antibody (clone CDLA47-1); Red = isotype control, Blue = CD47 antibody.

Immunofluorescent staining of paraformaldehyde-fixed human MCF7 cells with CD47 antibody (green, clone CDLA47-1) and DAPI nuclear stain (blue).

Quick Database Links

UniProt

Q08722

UniProt Details

−

No UniProt data available

Documents Download

Datasheet

Product Information

Download

Request a Document

Protocol Information

IHC-P

Immunohistochemistry Paraffin

View Protocol

FACS

Fluorescence-Activated Cell Sorting (FC, Flow cytometry)

View Protocol

Immunofluorescence

View Protocol

Find More Protocols