CD74 Mouse Monoclonal Antibody

SKU: orb500373

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Description

Research Area

CD Markers, Cell Biology, SARS /COVID-19

Images & Validation

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Item 1 of 3

Tested Applications	WB
Dilution Range	WB=1:500-2000
Reactivity	Human

Key Properties

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Antibody Type	Primary Antibody
Host	Mouse
Clonality	Monoclonal
Isotype	IgG2b
Clone No.	8D2
Immunogen	KLH conjugated synthetic peptide derived from human CD74 (1-232/296aaaa)
Target	CD74
Molecular Weight	34 kDa
Purification	Affinity purified by Protein G
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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CD 74; CD74 antigen; CD74 molecule; CLIP; DHLAG; gamma chain of class II antigens; HG2A_HUMAN; HLA class II histocompatibility antigen gamma chain; HLA DR antigens associated invariant chain; HLA DR gamma; HLA-DR antigens-associated invariant chain; HLA-DR-gamma; HLADG; HLADR antigens associated invariant chain; Ia antigen associated invariant chain; Ia antigen-associated invariant chain; Ia associated invariant chain; Ia gamma; Ii; Invariant polypeptide of major histocompatibility complex class II antigen associated; la-gamma; Major histocompatibility complex class II invariant chain; MHC HLA DR gamma chain; MHC HLA-DR gamma chain; p33; p35; Protein 41.

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Paraformaldehyde-fixed, paraffin embedded (Human appendix), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (CD74) Monoclonal Antibody, Unconjugated (orb500373) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Human spleen), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (CD74) Monoclonal Antibody, Unconjugated (orb500373) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human tonsil), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (CD74) Monoclonal Antibody, Unconjugated (orb500373) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Quick Database Links

Gene Symbol

CD74

UniProt

P04233

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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