Collagen IV antibody

SKU: orb2276390

Description

Collagen IV antibody validated for immunohistochemistry on 76 different Normal Tissues

Images & Validation

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Item 1 of 3

Tested Applications	IHC
Dilution range	1:100-1:200
Reactivity	Human
Application Notes	Positive Control: Colon: An at least moderate collagen IV immunostaining of basement membranes and a strong staining of vessels and the tunica muscularis mucosa should be seen. Negative Control: Colon: Collagen IV immunostaining must be absent in epithelial cells. Cellular Localization: Cytoplasmic and extracellular Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions. Read more...

Key Properties

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Host	Rabbit
Clonality	Monoclonal
Isotype	IgG
Clone No.	Biorbyt-704R
Immunogen	Purified human placental extract
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Disclaimer	For research use only

Alternative Names

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Arresten; BSVD; COL4A1; COL4A1 NC1 domain; COL4A2; COL4A3; COL4A4; COL4A5; collagen alpha-1(IV) chain; Collagen IV Alpha 1 Polypeptide; Collagen IV Alpha 2 Polypeptide; Collagen Type IV Alpha 2; Collagen Type IV Alpha 3; Collagen Type IV Alpha 4; Collagen Type IV Alpha 5; RATOR

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Collagen IV positive membranes surround pancreatic acini. Small blood vessels do also stain positive.

In the intestine collagen IV immunostaining involves basement membranes vessels of all sizes and smooth muscle cells of the lamina muscularis mucosae.

Papillary renal cell carcinoma showing a delicate collagen IV positive membrane between tumor cells and the stroma and an intense staining of vessels.

Quick Database Links

UniProt

P08572, P02462, P53420, P29400

UniProt Details

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No UniProt data available

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Protocol Information

IHC

Immunohistochemistry

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