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DDX58/RIG-1/RIG-I Antibody
SKU: orb1538154
Description
Images & Validation
−Item 1 of 2
| Tested Applications | ELISA, FC, IF, IHC, IHC-P |
|---|---|
| Dilution range | Flo (10 µg/ml U937 cells.), IF (10 µg/ml Strong expression of the protein seen in the cytoplasm of A431 and HeLa cells.), IHC, IHC-P (3.75 µg/ml), Peptide-ELISA (1:32000 Detection Limit) |
| Reactivity | Human |
Key Properties
−| Host | Goat |
|---|---|
| Clonality | Polyclonal |
| Immunogen | Peptide with sequence C-KDFHFEKIPFDPAE, from the C-Terminus of the protein sequence according to NP_055129.2. |
| Target | DDX58 / RIG-1 / RIG-I |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | TBS, pH 7.3, 0.02% Sodium Azide, 0.5% rAlbumin |
| Concentration | 0.5 mg/ml |
| Disclaimer | For research use only |
Alternative Names
−DDX58, RIG-1, RIG-I, RLR-1, Retinoic acid inducible gene I, RIG-I-like receptor 1, RIGI, DEAD box protein 58, RNA helicase RIG-I
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DDX58 / RIG-1 / RIG-I antibody immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).
DDX58 / RIG-1 / RIG-I antibody flow cytometric analysis of paraformaldehyde fixed U937 cells (blue line), permeabilized with 0.5% Triton.
Quick Database Links
Gene Symbol
DDX58 / RIG-1 / RIG-I
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Datasheet
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Protocol Information
IHC
Immunohistochemistry
IHC-P
Immunohistochemistry Paraffin
FC
Flow Cytometry
IF
Immunofluorescence
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
DDX58/RIG-1/RIG-I Antibody (orb1538154)
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