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DICER1 Antibody
SKU: orb1257224
Featured
Description
Images & Validation
−Item 1 of 5
| Tested Applications | IF, IHC, WB |
|---|---|
| Reactivity | Human, Mouse, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1200-1460 of human DICER1 (NP_085124.2). |
| Target | DICER1 |
| Molecular Weight | Observed: 250kDa |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Liquid |
| Concentration | batch dependent |
| Disclaimer | For research use only |
Alternative Names
−DCR1, Dicer, Dicer1e, GLOW, HERNA, K12H4.8-LIKE, MNG1, RMSE2, endoribonuclease Dicer, Dicer1, Dcr-1 homolog, dicer 1, double-stranded RNA-specific endoribonuclease, dicer 1, ribonuclease type III, helicase MOI, helicase with RNAse motif
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Western blot analysis of extracts of various cell lines, using DICER1 antibody (orb1257224) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human lung cancer using DICER1 antibody (orb1257224) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using DICER1 antibody (orb1257224) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using DICER1 antibody (orb1257224) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of L929 cells using DICER1 Polyclonal Antibody (orb1257224) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Quick Database Links
Gene Symbol
DICER1
UniProt
UniProt Details
− No UniProt data available
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
DICER1 Antibody (orb1257224)
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