DNAJC13 Antibody

SKU: orb1239876

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Description

DNAJC13 Antibody

Research Area

Cell Biology

Images & Validation

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Item 1 of 2

Tested Applications	ELISA, IHC-P, WB
Reactivity	Human, Mouse
Application Notes	DNAJC13 antibody can be used for detection of DNAJC13 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.Antibody validated: Western Blot in mouse samples and Immunohistochemistry in human samples. All other applications and species not yet tested.

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	DNAJC13 antibody was raised against an 18 amino acid peptide near the carboxy terminus of human DNAJC13.The immunogen is located within amino acids 1940 - 1990 of DNAJC13.
Target	DNAJC13
Molecular Weight	Predicted: 249 kDa Observed: 249 kDa
Purification	DNAJC13 antibody is affinity chromatography purified via peptide column.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	DNAJC13 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration	1 mg/mL
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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DNAJ homolog subfamily C member 13, receptor-mediated endocytosis 8, RME8

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WB Validation of DNAJC13 with Mouse Samples. Loading: 15 µg of lysate per lane.Detection Antibodies: DNAJC13, orb1239876, 1 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution, incubated at RT for 1 hr.

Immunohistochemistry of DNAJC13 in human heart tissue with DNAJC13 antibody at 5 µg/mL.

Quick Database Links

Gene Symbol

DNAJC13

UniProt

O75165

RefSeq (Protein):NP_056083

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_056083

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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