Eg5 (phospho Thr926) rabbit pAb Antibody

SKU: orb768904

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Description

Rabbit polyclonal antibody to Eg5 (phospho-Thr926)

Images & Validation

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Item 1 of 4

Tested Applications	ELISA, IF, IHC, WB
Dilution Range	Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications.
Reactivity	Human, Mouse, Rat

Key Properties

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Clonality	Polyclonal
Isotype	IgG
Immunogen	The antiserum was produced against synthesized peptide derived from human KIF11/Eg5 around the phosphorylation site of Thr926. AA range:892-941
Molecular Weight	119
Purification	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
Concentration	1 mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Anti-KIF11 antibody, anti-EG5 antibody, anti-KNSL1 antibody, anti-TRIP5 antibody, anti-Kinesin-like protein KIF11 antibody, anti-Kinesin-like protein 1 antibody, anti-Kinesin-like spindle protein HKSP antibody, anti-Kinesin-related motor protein Eg5 antibody, anti-Thyroid receptor-interacting protein 5 antibody, anti-TR-interacti antibody

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Eg5 (phospho Thr926) rabbit pAb Antibody

Western Blot analysis of COLO205 cells using Phospho-Eg5.

Western blot analysis of HELA KB SH-SY5Y 293T 3T3 lysis using Phospho-Eg5.

Immunohistochemical analysis of paraffin-embedded Human lung cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using KIF11/Eg5 (Phospho-Thr926) Antibody.

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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Immunofluorescence

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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