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ELF3 Antibody

SKU: orb1247565

Description

ELF3 Antibody

Research Area

Cancer Biology

Images & Validation

Tested ApplicationsELISA, WB
ReactivityHuman
Application Notes
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 48 kDa band observed in NCI-H460 lysate (calculated MW of 41.5kDa according to NP_004424.2). In transfected HEK293 transiently expressing ELF3 a band of approx. 40kDa is observed. This band is not observed in the non-transfected HEK293. Recommended for use at 2-4µg/mL.

Key Properties

Antibody TypePrimary Antibody
HostGoat
ClonalityPolyclonal
ImmunogenThe immunogen for this antibody is: C-SGWKEEEVLQSRN
TargetELF3
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration500 ug/mL
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

ELF3, ERT, E74-like factor 3 (ets domain transcription factor, epithelial-specific ), EPR-1, ESE-1, E74-like factor 3 (ets domain transcription factor), ets domain transcription factor, serine box (epithelial-specific), E74-like factor 3 (ETS domain transcription factor, serine box, epithelial-specific)

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ELF3 Antibody

orb1247565 staining (3 ug/ml) of NCI-H460 lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

ELF3 Antibody

HEK293 overexpressing ELF3 and probed with orb1247565 (mock transfection in first lane).

UniProt Details

No UniProt data available

NCBI Reference Sequences

Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product

Documents Download

Datasheet
Product Information
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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

ELF3 Antibody (orb1247565)

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0.1 mg
$ 920.00
DispatchUsually dispatched within 5-10 working days
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