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F(ab')₂ Fragment Goat Anti-Mouse IgG H&L, HRP conjugated
SKU: orb1151905
Description
Images & Validation
−Item 1 of 2
| Tested Applications | ELISA, IHC-Fr, IHC-P, WB |
|---|---|
| Dilution range | IHC-P=1:1000, WB=1:1000-20000, IHC-F=1:100-1000, ELISA=1:1000-20000 |
| Reactivity | Mouse |
Key Properties
−| Antibody Type | Secondary Antibody |
|---|---|
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Native Mouse IgG |
| Purification | Affinity purified by Protein G |
| Conjugation | HRP |
Storage & Handling
−| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
|---|---|
| Buffer/Preservatives | 10 mM TBS (pH=7.4) with 1% rAlbumin, 0.03% Proclin300 and 50% glycerol. |
| Concentration | 1.0 mg/ml |
| Disclaimer | For research use only |
Alternative Names
−F(ab)2 Fragment Goat Anti-Mouse IgG H&L (HRP); F(ab)2 Fragment Goat Anti-Mouse IgG H&L/HRP; F(ab)2 Fragment Goat Anti-Mouse IgG(H+L);
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Paraformaldehyde-fixed, paraffin embedded (rat pancreas), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (GLP-1 (1G9)) Monoclonal Antibody, Unconjugated (orb10721) at 1:200 overnight at 4°C, An HRP-conjugated secondary (orb1151905, 1:300 dilution) was used for 20min at 37°C, DAB was used as the chromogen.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IHC-Fr
Immunohistochemistry Frozen
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
F(ab')₂ Fragment Goat Anti-Mouse IgG H&L, HRP conjugated (orb1151905)
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