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F(ab')₂ Fragment Goat Anti-Mouse IgG H&L, Biotin conjugated

SKU: orb1974591

Description

F(ab')₂ Fragment Goat Anti-Mouse IgG H&L, Biotin conjugated

Images & Validation

Tested ApplicationsELISA, FC, ICC, IF, IHC-Fr, IHC-P, WB
Dilution rangeIHC-P=1:400-800, WB=1:1000-20000, IHC-F=1:100-1000, ELISA=1:1000-20000, ICC/IF=1:100-1000, IF=1:100-1000, Flow-Cyt=1:100-1000
ReactivityMouse

Related Conjugates & Formulations

Key Properties

Antibody TypeSecondary Antibody
HostGoat
ClonalityPolyclonal
IsotypeIgG
ImmunogenNative Mouse IgG
PurificationAffinity purified by Protein G, nonspecific adsorbed
ConjugationBiotin

Storage & Handling

StorageStore at -20°C for one year. Avoid repeated freeze/thaw cycles.
Buffer/Preservatives10 mM TBS (pH=7.4) with 1% rAlbumin, 0.03% Proclin300 and 50% glycerol.
DisclaimerFor research use only

Alternative Names

F(ab)2 Fragment Goat Anti-Mouse IgG H&L (Biotin); F(ab)2 Fragment Goat Anti-Mouse IgG H&L/Biotin; F(ab)2 Fragment Goat Anti-Mouse IgG(H+L)/Biotin;

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F(ab')₂ Fragment Goat Anti-Mouse IgG H&L, Biotin conjugated

Paraformaldehyde-fixed, paraffin embedded (human brain glioma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (S100B) Monoclonal Antibody, Unconjugated (orb500644) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

F(ab')₂ Fragment Goat Anti-Mouse IgG H&L, Biotin conjugated

Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Cytokeratin 8) Monoclonal Antibody, Unconjugated (orb500822) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

F(ab')₂ Fragment Goat Anti-Mouse IgG H&L, Biotin conjugated

Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (CK7) Monoclonal Antibody, Unconjugated (orb500618) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

F(ab')₂ Fragment Goat Anti-Mouse IgG H&L, Biotin conjugated

Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Cytokeratin 8) Monoclonal Antibody, Unconjugated (orb500822) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

F(ab')₂ Fragment Goat Anti-Mouse IgG H&L, Biotin conjugated

Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (S100B) Monoclonal Antibody, Unconjugated (orb500644) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

F(ab')₂ Fragment Goat Anti-Mouse IgG H&L, Biotin conjugated

Paraformaldehyde-fixed, paraffin embedded (mouse pancreas), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (GLP-1 (1G9)) Monoclonal Antibody, Unconjugated (orb10721) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

F(ab')₂ Fragment Goat Anti-Mouse IgG H&L, Biotin conjugated

Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (S100B) Monoclonal Antibody, Unconjugated (orb500644) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

F(ab')₂ Fragment Goat Anti-Mouse IgG H&L, Biotin conjugated

Paraformaldehyde-fixed, paraffin embedded (rat colon), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (alpha smooth muscle Actin) Monoclonal Antibody, Unconjugated (orb500841) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

F(ab')₂ Fragment Goat Anti-Mouse IgG H&L, Biotin conjugated

Paraformaldehyde-fixed, paraffin embedded (rat stomach), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (alpha smooth muscle Actin) Monoclonal Antibody, Unconjugated (orb500841) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

F(ab')₂ Fragment Goat Anti-Mouse IgG H&L, Biotin conjugated

Paraformaldehyde-fixed, paraffin embedded (rat uterus), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (alpha smooth muscle Actin) Monoclonal Antibody, Unconjugated (orb500841) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC-P
Immunohistochemistry Paraffin
View Protocol
IHC-Fr
Immunohistochemistry Frozen
View Protocol
FC
Flow Cytometry
View Protocol
IF
Immunofluorescence
View Protocol
ICC
Immunocytochemistry
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

F(ab')₂ Fragment Goat Anti-Mouse IgG H&L, Biotin conjugated (orb1974591)

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100 μl
$ 110.00
DispatchUsually dispatched within 2-3 weeks
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