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F(ab')2 Mouse IgG (H&L) Antibody
SKU: orb346783
Description
Images & Validation
−Item 1 of 1
| Tested Applications | ELISA, IHC, WB |
|---|---|
| Dilution range | ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000 |
| Reactivity | Mouse |
| Application Notes |
Key Properties
−| Antibody Type | Secondary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG F(ab')2 |
| Immunogen | Mouse IgG whole molecule |
| Purity | This product is a F(ab’)2 fragment of IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and pepsin digestion followed by chromatographic separation and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Mouse IgG and Mouse Serum. No reaction was observed against anti-Rabbit IgG F(c) or anti-Pepsin. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
| Concentration | 10.0 mg/mL |
| Disclaimer | For research use only |
Alternative Names
−Rabbit F(ab')2 Anti-MOUSE IgG Antibody, Rabbit Fab2 Anti Mouse IgG
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FcγRIV-dependent uptake and activation of kidney, but not dermal, macrophages by small immune complexes, ‘auto' antibodies, and NZWxBXSB serum. (H) TNF staining in kidney F4/80bright macrophages from wt mice 1 hour after i.v. injection of the indicated amounts of BSA, RaBSA, BSA;RaBSA, rabbit IgG (Ra-IgG), rabbit anti-mouse transferrin (Ra-mTransferrin) [p/n orb345374], rabbit anti-mouse albumin (Ra-mAlbumin) [p/n orb2652784], rabbit anti-mouse IgG (Ra-mIgG) [p/n orb347515] or Ra- mIgG (Fab')2 [p/n orb346783]. N = 4–5 mice per treatment.
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WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
F(ab')2 Mouse IgG (H&L) Antibody (orb346783)
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