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FHL1 Antibody
SKU: orb1257478
Featured
Description
Images & Validation
−Item 1 of 4
| Tested Applications | IF, IHC, WB |
|---|---|
| Reactivity | Human, Mouse |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 27-296 of human FHL1 (NP_001153171.1). |
| Target | FHL1 |
| Molecular Weight | Observed: 40kDa |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Liquid |
| Concentration | batch dependent |
| Disclaimer | For research use only |
Alternative Names
−FHL1, FHL1B, KYO-T, MGC111107, SLIM1, bA535K18.1, KYOT, SLIM, FHL-1, FHL1A, FLH1A, XMPMA, SLIM-1, SLIMMER
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Western blot analysis of extracts of various cell lines, using FHL1 antibody (orb1257478) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human thyroid cancer using FHL1 Antibody (orb1257478) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using FHL1 Antibody (orb1257478) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using FHL1 antibody (orb1257478). Blue: DAPI for nuclear staining.
Quick Database Links
Gene Symbol
FHL1
UniProt
UniProt Details
− No UniProt data available
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
FHL1 Antibody (orb1257478)
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