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FOXL2 Antibody

SKU: orb1248891

Description

FOXL2 Antibody

Images & Validation

Tested ApplicationsELISA, IHC, WB
ReactivityHuman, Mouse
Predicted ReactivityBovine, Rat
Application Notes
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 50kDa band observed in Human and Mouse Ovary lysates (calculated MW of 38.8kDa according to Human NP_075555.1 and 38.9kDa according to Mouse NP_036150.1). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration 0.3-1ug/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Rat Ovary for this particular batch.Immunohistochemistry:In paraffin embedded Human Ovary shows nuclear staining in select cells. Recommended concentration: 2-4ug/ml. Paraffin embedded Human Adrenal Gland. Recommended concentration: 5ug/ml.

Key Properties

Antibody TypePrimary Antibody
HostGoat
ClonalityPolyclonal
ImmunogenThe immunogen for this antibody is: C-DSKTGALHSRLDL
TargetFOXL2
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration500 ug/mL
DisclaimerFor research use only

Alternative Names

FOXL2, BPES, forkhead box L2, PFRK, BPES1, PINTO, forkhead transcription factor FOXL2, blepharophimosis, epicanthus inversus and ptosis, Blepharophimosis, epicanthus inversus, and ptosis 1, POF3

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FOXL2 Antibody

orb1248891 (2 μg/ml) staining of K562 cell lysate (A) + peptide (B) and HeLa cell lysate (C) + peptide (D) 35 μg protein in RIPA buffer). Detected by chemiluminescence.

FOXL2 Antibody

orb1248891 (2 ug/ml) staining of paraffin embedded Human Ovary. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

FOXL2 Antibody

orb1248891 (5 ug/ml) staining of paraffin embedded Human Adrenal Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

UniProt Details

No UniProt data available

NCBI Reference Sequences

Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

FOXL2 Antibody (orb1248891)

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0.1 mg
$ 920.00
DispatchUsually dispatched within 5-10 working days
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