Glypican-3 rabbit pAb Antibody

SKU: orb766811

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Description

Rabbit polyclonal antibody to Glypican-3

Images & Validation

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Tested Applications	ELISA, IF, IHC, WB
Dilution range	Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.
Reactivity	Human, Mouse, Rat

Key Properties

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Clonality	Polyclonal
Isotype	IgG
Immunogen	The antiserum was produced against synthesized peptide derived from the Internal region of human GPC3. AA range:461-510
Molecular Weight	70
Purification	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
Concentration	1 mg/ml
Disclaimer	For research use only

Alternative Names

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Anti-GPC3 antibody, anti-OCI5 antibody, anti-Glypican-3 antibody, anti-GTR2-2 antibody, anti-Intestinal protein OCI-5 antibody, anti-MXR7 antibody

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Western Blot analysis of HepG2 cells using Glypican-3 Polyclonal Antibody. Secondary antibody was diluted at 1:20000.

Immunohistochemical analysis of paraffin-embedded rat-liver, antibody was diluted at 1:100.

Western blot analysis of lysate from HepG2 cells, using GPC3 Antibody.

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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Immunofluorescence

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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