CD56/NCAM1 (isoform 2) Antibody

SKU: orb22474

Description

Goat polyclonal antibody to NCAM1

Research Area

Epigenetics, Infectious Diseases, Neuroscience

Images & Validation

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Item 1 of 2

Tested Applications	ELISA, FC, IHC, WB
Dilution range	ELISA: 1:32000, WB: 0.01-0.03 μg/ml, IHC-P: 5 μg/ml
Reactivity	Human
Predicted Reactivity	Bovine, Porcine, Rat
Application Notes	WB: Approx 170kDa band observed in Human Brain (Cerebellum, Cerebral and Frontal Cortex) lysates (calculated MW of 94.6kDa according to NP_851996.2). The observed molecular weight corresponds to the glycosylated form. Recommended concentration: 0.01-0.03 μg/ml.

Key Properties

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Host	Goat
Clonality	Polyclonal
Target	CD56 / NCAM1 (isoform 2)
Molecular Weight	94.6
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Disclaimer	For research use only

Alternative Names

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anti-CD56 antibody, anti-cell adhesion molecule, neural, 1 antibody, anti-MSK 39 antibody, anti-MSK39 antibody, anti-N-CAM-1 antibody, anti-NCAM 1 antibody, anti-NCAM antibody, anti-NCAM C antibody, anti-NCAM-1 antibody, anti-NCAM1 antibody, anti-NCAM1_HUMAN antibody, anti-NCAMC antibody, anti-Neural cell adhesion molecule 1 antibody, anti-Neural cell adhesion molecule NCAM antibody, anti-OTTHUMP00000235666 antibody

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orb22474 (0.01ug/ml) staining of Human Frontal Cortex lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemical staining of Human Adrenal Gland using NCAM1 antibody.

Quick Database Links

Gene Symbol

CD56 / NCAM1 (isoform 2)

NCBI Gene ID

4684

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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Flow Cytometry

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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