Goat anti-Complement factor H Antibody

SKU: orb19049

Description

Goat polyclonal antibody to CFH

Research Area

Cardiovascular Disease, Epigenetics, Immunology, Infectious Diseases

Images & Validation

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Item 1 of 2

Tested Applications	ELISA, FC, IHC, WB
Dilution range	ELISA: 1:32000, WB: 0.03-0.3 μg/ml, IHC-P: 5 μg/ml
Reactivity	Human
Application Notes	ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 170kDa band observed in human lung lysates (calculated MW of 139kDa according to NP_000177.2). Recommended concentration: 0.03-0.1 μg/ml.

Key Properties

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Host	Goat
Clonality	Polyclonal
Target	Complement factor H
Molecular Weight	139
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Disclaimer	For research use only

Alternative Names

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anti complement factor H antibody, anti HGNC:4883 antibody, anti CFH antibody, anti CFHL3 antibody, anti FHL1 antibody, anti HF antibody, anti HF1 antibody, anti HF2 antibody, anti HUS antibody, anti H factor 1 (complement) antibody, anti H factor 2 (complement) antibody, anti H factor-1 (complement) antibody, anti factor H-like 1 antibody, anti AHUS1 antibody, anti ARMD4 antibody, anti ARMS1 antibody, anti FH antibody, anti beta-1-H-globulin antibody, anti beta-1H antibody, anti complement factor H, isoform b antibody, anti factor H antibody, anti factor H-like 1 antibody

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orb19049 staining (0.03ug/ml) of human lung lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

orb19049 (5µg/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Quick Database Links

Gene Symbol

Complement factor H

NCBI Gene ID

3075

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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Flow Cytometry

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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