EBF1/COE1 Antibody

SKU: orb20040

Description

Goat polyclonal antibody to EBF1

Images & Validation

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Item 1 of 1

Tested Applications	ELISA, IP, WB
Dilution range	ELISA: 1:64000, WB: 0.3-0.1 μg/ml
Reactivity	Human, Mouse
Predicted Reactivity	Rat
Application Notes	ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Approx. 75kDa band observed in Human Adipose lysates (calculated MW of 64.5kDa according to NP_076870.1). Recommended concentration: 0.3-0.1 μg/ml.Experiment Notes: Anonymous customer used this product successfully in IP from induced 3T3-L1 expressing FLAG-EBF1.

Key Properties

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Host	Goat
Clonality	Polyclonal
Target	EBF1 / COE1
Molecular Weight	64.5
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Disclaimer	For research use only

Alternative Names

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anti EBF1 antibody, anti early B-cell factor 1 antibody, anti COE1 antibody, anti EBF antibody, anti O/E-1 antibody, anti OLF1 antibody, anti Collier, Olf and EBF transcription factor 1 antibody, anti early B-cell factor olfactory neuronal transcription factor 1 antibody

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orb20040 (0.3ug/ml) staining of Human Adipose lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Quick Database Links

Gene Symbol

EBF1 / COE1

NCBI Gene ID

1879

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Western Blot (IB, immunoblot)

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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Immunoprecipitation

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