Endophilin-A1/SH3GL2 Antibody

SKU: orb43524

Description

Goat polyclonal antibody to SH3GL2

Images & Validation

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Tested Applications	ELISA, IHC, WB
Dilution Range	ELISA: 1:64000, WB: 0.3-1 μg/ml, IHC-P: 5-10µg/ml
Reactivity	Human
Predicted Reactivity	Porcine
Application Notes	WB: Approx 38kDa band observed in Human Brain (Frontal Cortex) lysates (calculated MW of 40.0kDa according to NP_003017.1). Recommended concentration: 0.3-1µg/ml.. IHC: In paraffin embedded Human Cerebral Cortex shows staining of cytoplasm in some but not all neuronal cells. Recommended concentration, 5-10µg/ml.

Key Properties

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Host	Goat
Clonality	Polyclonal
Target	Endophilin-A1 / SH3GL2
Protein Sequence	SLEFPTGDSTQPN
Molecular Weight	40
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration	0.5 mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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anti bA335L15.1(SH3domain GRB2like 2) antibody, CNSA2 antibody, EENB1 antibody, endophilin antibody, endophilin1 antibody, FLJ20276 antibody, FLJ25015 antibody, OTTHUMP00000021084 antibody, SH3 domain protein 2A antibody, SH3 domaincontaining GRB2like protein 2 antibody, SH3D2A antibody, SH3domain GRB2like 2 antibody, SH3GL2 antibody, SH3P4 antibody

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Western blot analysis of Human Frontal Cortex lysate using SH3GL2 antibody

Immunohistochemical staining of Human Cerebral Cortex using SH3GL2 antibody

Quick Database Links

Gene Symbol

Endophilin-A1 / SH3GL2

NCBI Gene ID

6456

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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