Prolyl Endopeptidase/PREP Antibody

SKU: orb19233

Description

Goat polyclonal antibody to PREP

Research Area

Epigenetics

Images & Validation

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Item 1 of 1

Tested Applications	ELISA, IHC
Dilution range	ELISA: 1:16000, WB: 0.3 μg/ml, IHC-P: 3.75 μg/ml
Reactivity	Human
Predicted Reactivity	Bovine, Canine, Mouse, Porcine, Rat
Application Notes	ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Preliminary experiments gave an approx 45kDa band in Human Brain, Lymph Node, Spleen and Testis lysates after 0.3 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 80.7kDa according to NP_002717.3. The 45kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported Read more...

Key Properties

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Host	Goat
Clonality	Polyclonal
Target	Prolyl Endopeptidase / PREP
Molecular Weight	80.7
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Disclaimer	For research use only

Alternative Names

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anti PREP antibody, anti prolyl endopeptidase antibody, anti HGNC:9358 antibody, anti MGC16060 antibody, anti PE antibody, anti PEP antibody, anti OTTHUMP00000040498 antibody, anti dJ355L5.1 (prolyl endopeptidase) antibody, anti post-proline cleaving enzyme antibody

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Immunohistochemical staining of Human Kidney using PREP antibody

Quick Database Links

Gene Symbol

Prolyl Endopeptidase / PREP

NCBI Gene ID

5550

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

IHC

Immunohistochemistry

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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