Ramp1 (C-Term., mouse) Antibody

SKU: orb19922

Description

Goat polyclonal antibody to Ramp1

Images & Validation

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Item 1 of 1

Tested Applications	ELISA, WB
Dilution range	ELISA: 1:64000, WB: 0.3-1 μg/ml
Reactivity	Rat
Predicted Reactivity	Human, Mouse
Application Notes	ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Experiments gave bands at approx. 18kDa and 40kDa in Rat Brain lysate after 0.3 μg/ml antibody staining. The appearance of a band with much higher molecular weight corresponds to earlier findings in literature with different antibodies (Hilairet, J Biol Chem. 2001 Nov 9;276(45):42182-90.; PMID: 11535606). This protein has a calculated MW of 16.9kDa according to Mouse NP_058590.1 and 16.8kDa according to Rat NP_113833.1. Recommended concentration: 0.3-1 μg/ml. Read more...

Key Properties

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Host	Goat
Clonality	Polyclonal
Target	Ramp1 (C-Term., mouse)
Molecular Weight	16.9; 11.5
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Disclaimer	For research use only

Alternative Names

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anti Ramp1 antibody, anti receptor (calcitonin) activity modifying protein 1 antibody, anti 9130218E19Rik, MGC38240 antibody, anti receptor-activity modifying protein 1 antibody

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orb19922 (0.3ug/ml) staining of Rat Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Quick Database Links

Gene Symbol

Ramp1 (C-Term., mouse)

NCBI Gene ID

10267

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Western Blot (IB, immunoblot)

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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