Goat IgG Antibody

SKU: orb346106

Description

Goat IgG Antibody

Research Area

Protein Biochemistry

Images & Validation

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Item 1 of 2

Tested Applications	SDS-PAGE
Application Notes	Goat IgG whole molecule has been tested in SDS-Page and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments.

Key Properties

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Source	Goat
Biological Origin	Goat
Isotype	IgG
Purity	Goat IgG whole molecule was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Goat IgG whole molecule assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat IgG and anti-Goat Serum.
Conjugation	Unconjugated

Storage & Handling

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Storage	Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Goat IgG whole molecule is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Form/Appearance	Lyophilized
Buffer/Preservatives	Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Concentration	10 mg/ml
Disclaimer	For research use only

Alternative Names

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Goat immunoglobulin G

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SDS-PAGE of Goat IgG Whole Molecule Rhodamine Conjugated (p/n orb346100). Lane M: 5 µl Opal Prestained Marker. Lane 1: Reduced Goat IgG Whole Molecule Rhodamine Conjugated (p/n orb346100). Lane 2: Reduced Goat IgG F(c) Fragment (p/n orb2652765). Lane 3: Reduced Goat IgG F(ab) Fragment (p/n orb2652763). Lane 4: Reduced Goat IgM Whole Molecule (p/n orb346110). Load: 1 µg for IgG, F(c) and F(ab); 3 µg for IgM. Predicted/Observed size: IgG at 50 and 25 kDa; F(c) at 25 kDa; F(ab) at 25 kDa; IgM at 70 and 23 kDa. Observed F(c) Fragment migrates slightly higher.

SDS-PAGE of Goat IgG Whole Molecule. Lane 1: Reduced Goat IgG Whole Molecule. Lane 2: 3 µl OPAL Pre-stained Marker. Lane 3: Non-reduced Goat IgG Whole Molecule. Load: 1 µg per lane. Predicted/Observed size: Non-reduced at 160 kDa/observed at 180-200 kDa; Reduced at 55, 25 kDa. Non-reduced migrates at slightly higher molecular weight.

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Protocol Information

SDS-PAGE

Sodium Dodecyl Sulphate PolyAcrylamide Gel Electrophoresis

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