gp96 Antibody / GRP94 / HSP90B1

SKU: orb2639298

Description

Recognizes a protein of 94kDa, which is identified as the glucose-regulated protein 94 (grp94) and also tumor rejection antigen (gp96). Gp96 shows a high degree of sequence homology with the heat shock protein 90 (hsp90). This mAb is highly specific and shows minimal cross-reaction with other members of the HSP90 family. Grps are a class of proteins unresponsive to heat shock and are induced by glucose deprivation. Gp96 has been briefly studied as a prognostic factor in breast cancer.

Images & Validation

−

Item 1 of 3

Tested Applications	FACS, IF, IHC-P
Dilution range	Flow cytometry: 1-2ug/10^6 cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Reactivity	Human
Application Notes	Optimal dilution of the gp96 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

−

Antibody Type	Primary Antibody
Host	Rat
Clonality	Monoclonal
Isotype	Rat IgG2a, kappa
Clone No.	SPM249
Immunogen	Purified glucose regulated protein 94 from chicken oviducts was used as the immunogen for the gp96 antibody.
Purification	Protein G affinity chromatography
Conjugation	Unconjugated

Storage & Handling

−

Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Disclaimer	For research use only

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

IHC analysis of formalin-fixed, paraffin-embedded human breast carcinoma stained with gp96 antibody (SPM249).

Immunofluorescent staining of permeabilized human HepG2 cells with gp96 antibody (clone SPM249, green) and Reddot nuclear stain (red).

Flow cytometry testing of PFA-fixed human HepG2 cells with gp96 antibody (clone SPM249); Red = isotype control, Blue = gp96 antibody.

Quick Database Links

UniProt

P14625

UniProt Details

−

No UniProt data available

Documents Download

Datasheet

Product Information

Download

Request a Document

Protocol Information

IHC-P

Immunohistochemistry Paraffin

View Protocol

FACS

Fluorescence-Activated Cell Sorting (FC, Flow cytometry)

View Protocol

Immunofluorescence

View Protocol

Find More Protocols