GST Antibody (FITC)

SKU: orb413651

Description

Mouse monoclonal antibody against GST conjugated to FITC

Research Area

Cell Biology, Disease Biomarkers, Metabolism Research, Signal Transduction

Images & Validation

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Tested Applications	ELISA, WB
Dilution range	WB (1:1000); ELISA (1:800)
Reactivity	All
Application Notes	A 1:1000 dilution of SMC-549 was sufficient for detection of GST in 1 µg of Purified GST tagged PmtA by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.

Key Properties

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Host	Mouse
Clonality	Monoclonal
Isotype	IgG1
Clone No.	300
Immunogen	Glutathione-S-Transferase (GST) tagged protein
Target	GST
Molecular Weight	26 kDa
Purification	Protein G Purified
Conjugation	FITC

Storage & Handling

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Storage	Conjugated antibodies should be stored according to the product label
Buffer/Preservatives	640.91mM DMSO, 136.36 mM Ethanolamine, 126.89 mM chlorides, 9.09mM phosphates, 9.09mM NaHCO3
Concentration	1 mg/ml
Disclaimer	For research use only

Alternative Names

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Glutathione S Transferase, Glutathione S-transferase class-mu 26 kDa isozyme, GST, Sj26 antigen, SjGST, Glutathione-S-Transferase, GST tag, GST tagged protein, GST fusion protein, GST protein

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Western Blot analysis of Schistosoma japonicum Purified GST showing detection of 26 kDa GST protein using Mouse Anti-GST Monoclonal Antibody, Clone 3E2. Lane 1: Molecular Weight Ladder (MW). Lane 2: Purified Pseudomonas aeruginosa Metallothionein (PmtA) GST tagged protein. Lane 3: GST. Load: 1 μg. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-GST Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG:HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 26 kDa.

Quick Database Links

Gene Symbol

GST

UniProt

P08515

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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