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Hemoglobin beta C Antibody
SKU: orb420241
Description
Images & Validation
−Item 1 of 2
| Tested Applications | ELISA, WB |
|---|---|
| Dilution Range | ELISA: 1:20,000, WB: 1ug/mL |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Clone No. | 15C2.C11.F2.G11 |
| Immunogen | Anti-Hemoglobin beta C Monoclonal Antibody was produced in mice by repeated immunizations with synthetic peptide corresponding to amino acid residues near the N-terminus of Hb β-subunit conjugated to KLH. |
| Purity | This protein A purified mouse monoclonal antibody reacts specifically with human HbC beta c-variant isoform. Anti-HbC is purified from tissue culture supernatant by protein A purification. Blast analysis shows 100% homology to Human, Pan troglodytes, Pan paniscus, Gorilla gorilla gorilla, and Hylobates lar. This antibody does not react with the HbA, HbS, HbF, or HbA-2 isoforms. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing. |
|---|---|
| Form/Appearance | Liquid (sterile filtered) |
| Buffer/Preservatives | Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Concentration | 1.0 mg/mL |
| Expiration Date | 12 months from date of receipt. |
| Dry Ice Shipping | Please note: This product requires shipment on dry ice. A dry ice surcharge will apply. |
| Disclaimer | For research use only |
Alternative Names
−mouse anti-HbC antibody, mouse anti-hemoglobin antibody, Hemoglobin beta subunit C variant, HbBc, HbC, HbC Antibody, LVV-hemorphin-7, Spinorphin, Beta-globin, Hemoglobin beta chain, Sickle Cell Disease (SCD)
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ELISA results of purified Monoclonal anti-HbC antibody tested against BSA-conjugated peptide of HbC peptide. (Purple Line). Each well was coated in duplicate with 0.1 µg of conjugate. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Blocking buffer, HRP conjugated (p/n orb347544) and TMB substrate (p/n orb348657).
Western Blot of Mouse Anti-Hemoglobin beta C Antibody. Lane 1: Molecular Weight Ladder. Lane 2: HbA peptide conjugated to BSA. Lane 3: HbA-2 peptide conjugated to BSA. Lane 4: HbC peptide conjugated to BSA. Lane 5: HbF peptide conjugated to BSA. Lane 6: HbS peptide conjugated to BSA. Lane 7: BSA alone. Load: 50 ng per lane. Primary antibody: Anti-HbC antibody at 1 µg/ml overnight at 4°C. Secondary antibody: Rabbit Anti-Mouse secondary antibody at 1:40000 for 30 min at RT. Block: orb348644 for 30 min RT. Predicted/Observed: Reactivity seen in Lane 4 specific to HbC only.
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Protocol Information
WB
Western Blot (IB, immunoblot)
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
Hemoglobin beta C Antibody (orb420241)
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