HSP70L rabbit pAb Antibody

SKU: orb765450

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Description

Rabbit polyclonal antibody to HSP70

Images & Validation

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Tested Applications	ELISA, IF, IHC, WB
Dilution range	Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.
Reactivity	Human, Mouse, Rat

Key Properties

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Clonality	Polyclonal
Isotype	IgG
Immunogen	The antiserum was produced against synthesized peptide derived from human HSPA1L. AA range:221-270
Molecular Weight	70
Purification	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
Concentration	1 mg/ml
Disclaimer	For research use only

Alternative Names

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Anti-HSPA1L antibody, anti-Heat shock 70 kDa protein 1-like antibody, anti-Heat shock 70 kDa protein 1L antibody, anti-Heat shock 70 kDa protein 1-Hom antibody, anti-HSP70-Hom antibody, anti-HSPA1A antibody, anti-HSPA1 antibody, anti-HSPA1B antibody, anti-Heat shock 70 kDa protein 1A/1B antibody, anti-Heat shock 70 kDa protein 1/ antibody

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Western Blot analysis of various cells using HSP70 Polyclonal Antibody diluted at 1:1000.

Western Blot analysis of MCF-7 cells using HSP70 Polyclonal Antibody diluted at 1:1000.

Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive control (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Western blot analysis of lysates from MCF-7 cells and HUVEC cells, using HS71L Antibody. The lane on the right is blocked with the synthesized peptide.

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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Immunofluorescence

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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