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Human STEAP1

SKU: orb3002171

Description

Recombinant Human STEAP1 is produced by our Mammalian expression system and the target gene encoding Glu2-Leu339 is expressed with a Flag tag at N-terminus and a 6His tag at C terminus.Note: For the preservation of the proper conformation and biological activity(including ELISA, BLI, SPR applications) of Recombinant Human STEAP1(N-Flag, C-6His)(Cat. No.:C48A-A), a specific buffer 50×GDN Buffer(Cat. No.:RB03) is necessary.

Images & Validation

Key Properties

Expression SystemHuman Cells
Biological ActivityELISA: Immobilized Anti-Human STEAP1 Antibody (VAN_bio, Research Grade) (Cat#orb2992566) at 2μg/ml (100 μl/well) can bind Recombinant Human STEAP1 (N-Flag, C-6His) (Cat#orb3002171).The EC50 of Recombinant Human STEAP1 (N-Flag, C-6His)(Cat#orb3002171) is 79.33ng/ml.(Regularly tested).. BLI: Loaded Anti-Human STEAP1 Antibody (VAN_bio, Research Grade) (Cat#orb2992566) on Pro-A Biosensor, can bind Recombinant Human STEAP1 (N-Flag, C-6His) (Cat#orb3002171) with an affinity constant of 5.89 nM as determined in BLI assay. (Regularly tested)
TagN-Flag, C-6His
Molecular WeightPredicted: 41.7kDa. Observed: (36-44)&(69-85)kDa, reducing conditions
Expression RegionGlu2-Leu339
PuritySDS-PAGE: Greater than 90% as determined by reducing SDS-PAGE. (QC verified)
Endotoxins<1 EU/µg as determined by LAL test.

Storage & Handling

StorageStore at ≤-70°C, stable for 6 months after receipt.Store at ≤-70°C, stable for 3 months under sterile conditions after opening.Please minimize freeze-thaw cycles.
Form/AppearanceSupplied as a 0.2 μm filtered solution of 50mM HEPES-Na, 150mM NaCl, 5%Glycerol, 0.08%GDN, pH 7.5.
DisclaimerFor research use only

Alternative Names

STEAP1 protein; Six-transmembrane epithelial antigen of prostate 1; STEAP1

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Human STEAP1 (orb3002171)

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