IL-4-TF tag mRNA-LNP

Interleukin 4 (IL-4), encoded by the IL4 gene, is a pleiotropic cytokine known primarily for its role in type 2 immunity. IL-4 is mainly produced by mast cells, Th2 cells, eosinophils and basophils. By binding to its receptor IL-4R, this cytokine exerts many biological effects, including stimulation of proliferation of activated B and T cells, and differentiation of B cells into plasma cells. IL4 is considered a canonical regulatory cytokine because alternative activation of myeloid cells was first observed after stimulation with Th2 lymphocyte-derived IL4. IL4 is structurally and functionally related to another immunomodulatory cytokine, IL13. IL-4 has been found to be increased in breast, prostate, lung, renal cell, and other types of cancer, and IL-4R is overexpressed in many types of cancer, suggesting that IL-4 has a significant impact on tumor progression. This product is designed as a tool for the delivery and expression of TF-tagged human IL-4 mRNA for research. The product leverages the lipid nanoparticle (LNP) technology platform for simple and efficient delivery of hIL4-TF mRNA to a variety of mammalian cells in vitro and in vivo. The LNPs used are formulated with SM-102, DSPC, cholesterol and DMG-PEG2000 at an optimal molar concentration for a high rate of encapsulation and efficient mRNA delivery. The hIL4-TF protein is about 19 kD, consisting of hIL4 and a C-terminal TF tag (15 amino acids). The GenPept accession number for hIL4 (153 amino acids) is NP_000580.