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MC4 Receptor Polyclonal Antibody
SKU: orb1421140
Description
Research Area
Epigenetics, GPCR, Neuroscience, Signaling Pathways
Images & Validation
−Item 1 of 5
| Tested Applications | ELISA, ICC, IF, IHC-Fr, WB |
|---|---|
| Reactivity | Rat |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Melanocortin receptor 4, MC4-R, MC4R
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Sample: Embryo (Mouse) Lysate at 40 ug. Primary: Anti- MC4 Receptor at 1/1000 dilution. Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution. Predicted band size: 37 kD. Observed band size: 52 kD.
Sample: MCF-7 Cell (Human) Lysate at 40 ug. Primary: Anti-MC4 Receptor at 1/300 dilution. Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution. Predicted band size: 37 kD. Observed band size: 40 kD.
Positive control: RSC96. Isotype Control Antibody: Rabbit IgG; Secondary Antibody: Goat anti-rabbit IgG-FITC, Dilution: 1: 100 in 1 X PBS containing 0.5% BSA; Primary Antibody Dilution: 6 µg in 100 µL 1X PBS containing 0.5% BSA.
Blank control: 293T (fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice).Primary Antibody: Rabbit Anti-MC4 Receptor /AF488 Conjugated antibody, Dilution: 1 µg in 100 µL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG/AF488 (orange), used under the same conditions.
Blank control: 293T (fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice). Primary Antibody: Rabbit Anti-MC4 Receptor /AF488 Conjugated antibody, Dilution: 1 µg in 100 µL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG/AF488 (orange), used under the same conditions.
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UniProt
UniProt Details
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-Fr
Immunohistochemistry Frozen
IF
Immunofluorescence
ICC
Immunocytochemistry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
MC4 Receptor Polyclonal Antibody (orb1421140)
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