Mouse IgG (H&L) Antibody Alkaline Phosphatase Conjugated Pre-Adsorbed

SKU: orb347568

Description

Mouse IgG (H&L) antibody (Alkaline Phosphatase)

Images & Validation

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Item 1 of 1

Tested Applications	ELISA, IHC, WB
Dilution range	ELISA: 1:9,800, IHC: 1:100 - 1:500, WB: 1:500 - 1:2,000
Reactivity	Mouse
Application Notes	Anti-Mouse IgG Alkaline Phosphatase conjugate has been tested by ELISA and western blot and is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody-based enzymatic assays requiring lot-to-lot consistency.

Key Properties

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Antibody Type	Secondary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Mouse IgG whole molecule
Purity	This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose followed by solid phase adsorption to remove unwanted reactivities and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Mouse IgG and Mouse Serum. No reactivity was observed against Human serum proteins.
Conjugation	AP

Storage & Handling

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Storage	Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Form/Appearance	Liquid (sterile filtered)
Buffer/Preservatives	0.01% (w/v) Sodium Azide
Concentration	1.0 mg/mL
Disclaimer	For research use only

Alternative Names

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rabbit anti-Mouse IgG Antibody alkaline phosphatase conjugation, rabbit anti-Mouse IgG alk phos conjugated Antibody

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Mouse IgG (H&L) Antibody Alkaline Phosphatase Conjugated Pre-Adsorbed

Western Blot of Alkaline Phosphatase Conjugated Rabbit anti-Mouse IgG antibody. Lane 1: Mouse IgG. Lane 2: none. Load: 100 ng per lane. Primary antibody: none. Secondary antibody: Alkaline Phosphatase mouse secondary antibody at 1:1000 for 60 min at RT. Block: orb348637 for 30 min RT. Predicted/Observed size: 55 kDa, 28 kDa for Mouse IgG. Other band(s): none.

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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