Mouse Soluble receptor activator of nuclear factor-kB ligand (sRANKL) ELISA Kit

SKU: orb3147768

Description

This Mouse Soluble receptor activator of nuclear factor-kB ligand (sRANKL) ELISA Kit employs a two-site sandwich ELISA to quantitate TNFSF11.

Images & Validation

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Tested Applications

ELISA

Application Notes

This Mouse Soluble receptor activator of nuclear factor-kB ligand (sRANKL) ELISA Kit employs a two-site sandwich ELISA to quantitate TNFSF11 in samples. An antibody specific for TNFSF11 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTNFSF11 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNFSF11 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNFSF11 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Key Properties

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Reactivity	Mouse
Sample Types	Cell culture supernatants#Serum#Plasma#Other biological fluids
Range	Please inquire
Sensitivity	Please inquire

Storage & Handling

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Storage	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Expiration Date	6 months from date of receipt.
Hazard Information	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Disclaimer	For research use only

Alternative Names

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TNFSF11; CD254; ODF; OPGL; OPTB2; RANKL; TRANCE; hRANKL2; sOdf; OTTHUMP00000178585; TNF-related activation-induced cytokine; osteoclast differentiation factor; osteoprotegerin ligand; receptor activator of nucle

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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