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CD56 Antibody
SKU: orb534487
Description
Research Area
Epigenetics, Infectious Diseases
Images & Validation
−Item 1 of 5
| Tested Applications | IF, IHC-P, WB |
|---|---|
| Dilution range | Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Western blot: 1-2ug/ml |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | Mouse IgG1, kappa |
| Clone No. | 56C04, also called 123A8 |
| Immunogen | A membrane preparation of small cell lung carcinoma was used as the immunogen for this CD56 antibody. This mAb reacts with an extracellular domain (close to transmembrane) of CD56/NCAM. |
| Purification | Protein G affinity chromatography |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Disclaimer | For research use only |
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Western blot testing of human brain tissue with CD56 antibody (clone 56C04/123A8). Predicted molecular weight: ~110 kDa (soluble fragment), ~120/125 kDa (GPI-anchored), 140/180 kDa (transmembrane isoforms).
IHC staining of FFPE human pancreas tissue with CD56 antibody (clone 56C04/123A8). HIER: boil tissue sections in pH9 EDTA buffer for 10-20 min followed by cooling at RT for 20 min.
IHC staining of FFPE human thyroid tissue with CD56 antibody (clone 56C04/123A8). HIER: boil tissue sections in pH9 EDTA buffer for 10-20 min followed by cooling at RT for 20 min.
IHC staining of FFPE human colon tissue with CD56 antibody (clone 56C04/123A8). HIER: boil tissue sections in pH9 EDTA buffer for 10-20 min followed by cooling at RT for 20 min.
SDS-PAGE analysis of purified, BSA-free CD56 antibody (clone 56C04/123A8) as confirmation of integrity and purity.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IF
Immunofluorescence
CD56 Antibody (orb534487)
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