Neu (phospho Tyr877) rabbit pAb Antibody

SKU: orb764239

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Description

Rabbit polyclonal antibody to Neu (phospho-Tyr877)

Images & Validation

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Item 1 of 4

Tested Applications	ELISA, IF, IHC, IP, WB
Dilution range	Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunoprecipitation: 2-5 ug/mg lysate. ELISA: 1/10000. Not yet tested in other applications.
Reactivity	Human, Mouse, Rat

Key Properties

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Clonality	Polyclonal
Isotype	IgG
Immunogen	The antiserum was produced against synthesized peptide derived from human HER2 around the phosphorylation site of Tyr877. AA range:851-900
Molecular Weight	180
Purification	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
Concentration	1 mg/ml
Disclaimer	For research use only

Alternative Names

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Anti-ERBB2 antibody, anti-HER2 antibody, anti-MLN19 antibody, anti-NEU antibody, anti-NGL antibody, anti-Receptor tyrosine-protein kinase erbB-2 antibody, anti-Metastatic lymph node gene 19 protein antibody, anti-MLN 19 antibody, anti-Proto-oncogene Neu antibody, anti-Proto-oncogene c-ErbB-2 antibody, anti-Tyrosine kinase-type cell surface rec antibody

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Neu (phospho Tyr877) rabbit pAb Antibody

Western Blot analysis of various cells using Phospho-Neu (Y877) Polyclonal Antibody diluted at 1:500.

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using HER2 (Phospho-Tyr877) Antibody.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using HER2 (Phospho-Tyr877) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from HepG2 cells and HeLa cells, using HER2 (Phospho-Tyr877) Antibody. The lane on the right is blocked with the phospho peptide.

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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Immunofluorescence

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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Immunoprecipitation

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