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p16-INK4a (Phospho-Ser152) rabbit pAb Antibody
SKU: orb767331
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Description
Images & Validation
−Item 1 of 4
| Tested Applications | ELISA, IF, IHC, WB |
|---|---|
| Dilution Range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications. |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human p16-INK4a around the phosphorylation site of Ser152. AA range:107-156 |
| Molecular Weight | 20 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
| Concentration | 1 mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Anti-CDKN2A antibody, anti-CDKN2 antibody, anti-MTS1 antibody, anti-Cyclin-dependent kinase inhibitor 2A antibody, anti- isoforms 1/2/3 antibody, anti-Cyclin-dependent kinase 4 inhibitor A antibody, anti-CDK4I antibody, anti-Multiple tumor suppressor 1 antibody, anti-MTS-1 antibody, anti-p16-INK4a antibody, anti-p16-INK4 antibody, anti-p16INK4A antibody
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Western Blot analysis of JK cells using Phospho-p16 (S326) Polyclonal Antibody diluted at 1:500.
Western Blot analysis of 22RV1 HELA cells using Phospho-p16 (S326) Polyclonal Antibody diluted at 1:500.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using p16-INK4a (Phospho-Ser152) Antibody. The picture on the right is blocked with the phospho peptide.
Western blot analysis of lysates from HeLa cells treated with EPO 20U/ml 15', using p16-INK4a (Phospho-Ser152) Antibody. The lane on the right is blocked with the phospho peptide.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
p16-INK4a (Phospho-Ser152) rabbit pAb Antibody (orb767331)
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