Phospho-MET (Tyr1234/1235) (6F11) rabbit mAb Antibody

SKU: orb1946193

Description

c-Met, also called tyrosine-protein kinase MET or hepatocyte growth factor receptor (HGFR), has tyrosine kinase activity (1). MET is a single pass tyrosine kinase receptor essential for embryonic development, organogenesis and wound healing. Normally, MET is expressed only in stems cells and progenitor cells but excessive expression of MET/HGFR and its autocrine activation by co-expression of hepatocyte growth factor (HGF) ligand are implicated in oncogenesis (2,3). Aberrantly activated MET leads to tumor growth, angiogenesis, and cancer metastasis and is correlated with poor prognosis. Abnormal activation of MET is observed in various human malignancies, such as kidney, liver, stomach, breast, and brain. MET activation by HGF induces MET kinase catalytic activity and leads to phosphorylation at Tyr 1234 and Tyr 1235.

Images & Validation

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Item 1 of 4

Tested Applications	FC
Dilution range	1µg/mL - 0.001µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
Reactivity	Human, Mouse

Key Properties

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Host	Rabbit
Clonality	Monoclonal
Isotype	Rabbit IgG1k
Clone No.	MetY12341235-6F11
Immunogen	A synthetic phospho-peptide corresponding to residues surrounding Tyr1234/Tyr1235 of human phospho Met
Purification	Protein A+G
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% rAlbumin
Concentration	0.5 mg/mL
Disclaimer	For research use only

Alternative Names

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AUTS9; c-Met; Hepatocyte growth factor receptor; HGF receptor; HGF/SF receptor; HGFR; MET; met proto-oncogene (hepatocyte growth factor receptor); met proto-oncogene tyrosine kinase; oncogene MET; Proto-oncogene c-Met; RCCP2; Scatter factor receptor; SF receptor; Tyrosine-protein kinase Met

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Phospho-MET (Tyr1234/1235) (6F11) rabbit mAb Antibody

Flow cytometric analysis of Ramos cells secondary antibody only negative control (blue) untreated (red) treated with FBS + pervanadate (green) treated plus blocked by non-phospho peptide (violet) or treated plus blocked by phospho-peptide (brown) using 0.01 ug/mL Phospho-MET (Y1234/1235) antibody METY12341235-6F11 0.01 ug/mL. Cat. #orb1946193.

Flow cytometric analysis of NIH3T3 cells secondary antibody only negative control (blue) or 0.1 μg/mL of isotype control Cat. #orb1946241 (orange) or treated with imatinib (red) or with pervanadate (green) using Phospho-MET (Y1234/1235) antibody METY12341235-6F11 at 0.1 μg/mL. Cat #orb1946193.

Flow cytometric analysis of Ramos cells secondary antibody only negative control (blue) or untreated (red) or treated with pervanadate (green) using 0.005 ug/mL Phospho-MET (Y1234/1235) antibody METY12341235-6F11. Cat. # orb1946193.

Flow cytometric analysis of Ramos cells secondary only negative control (blue) or untreated (red) or treated with FBS + pervanadate (green) using Phospho-Met (Tyr1234/1235) antibody MetY12341235-6F11 (Cat. #orb1946193) at 0.01ug/mL or Company C antibody at 0.2ug/mL (manufacturer's recommended concentration).

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UniProt

P08581

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No UniProt data available

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Flow Cytometry

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