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PKR (PRKR) Antibody (N-term)
SKU: orb39034
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Description
Research Area
Cell Biology, Immunology & Inflammation, Infectious Disease & Virology, Signal Transduction
Images & Validation
−Item 1 of 3
| Tested Applications | IHC-P, WB |
|---|---|
| Dilution range | WB: 1:1000, IHC-P: 1:50-100 |
| Reactivity | Human, Mouse |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Clone No. | RB5281, RB5282 |
| Immunogen | Synthetic Peptide |
| Target | EIF2AK2 |
| Molecular Weight | 62094 |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Disclaimer | For research use only |
Alternative Names
−Anti-"Interferon-induced antibody, anti-double-stranded RNA-activated protein kinase antibody, anti-Interferon-inducible RNA-dependent protein kinase antibody, anti-p68 kinase antibody, anti-P1/eIF-2A protein kinase antibody, anti-EIF2AK2 antibody, anti-PKR" antibody
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Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
The anti-PRKR Pab is used in Western blot to detect PRKR in mouse liver tissue lysate (Lane 1) and HepG2 cell lysate (Lane 2).
Western blot analysis of EIF2AK2 (arrow) using PRKR Antibody (N-term).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the EIF2AK2 gene (Lane 2).
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_001129124.1, NP_002750.1, NP_001129123.1 |
|---|
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
PKR (PRKR) Antibody (N-term) (orb39034)
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